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If you are interest to learn about the principle and method of ELISA, is your right resource online. Enzyme-linked immunosorbent assay (ELISA) is a method of target antigen (or antibody) capture in samples using a specific antibody (or antigen), and of target molecule detection/quantitation using an enzyme reaction with its substrate. The Boster Bio protocol for ELISA offers a step-by-step overview of the procedure. At you will find all the information related to Elisa.

An enzyme-linked immunosorbent assay, also called ELISA or EIA, is a test that detects and measures antibodies in your blood. This test can be used to determine if you have antibodies related to certain infectious conditions. An ELISA sample preparation test may be used to diagnose: HIV, which causes AIDS.

Types of ELISA

Frequently there are 3 types of ELISA on the basis of binding structure between the Antibody and Antigen.

  • Indirect ELISA
  • Sandwich ELISA
  • Competitive ELISA

Sandwich Elisa Principle is based on Antigen that can be detected by sandwich ELISA. In this technique, antibody is coated on the microtiter well. A sample containing antigen is added to the well and allowed to react with the antibody attached to the well, forming antigen-antibody complex. After the well is washed, a second enzyme-linked antibody specific for a different epitope on the antigen is added and allowed to react with the bound antigen.

Sandwich Elisa Protocol comprises of prepare a surface to which a known quantity of antibody is bound. Add the antigen-containing sample to the plate and incubate the plate at 37°c. Wash the plate, so that unbound antigen is removed.

About Antibody Elisa:

Modern medicine recognizes the complications of treating infectious diseases. It is realized that early diagnosis of diseases is extremely important these days.

Connecting particular proteins to specific disease continues to pose challenges to science even today, though a lot of developments have taken place in past. ELISA can be used to probe numerous diseases in several organisms. The ELISA is a simple test that produces dependable data in a short span of time. ELISA or Enzyme- link immunosorbent assay is the technique used to detect the presence of antibodies in the blood. In some instances, this test is not just for patience but also for plant pathology and quality control in various industries.